Use of the DCB Technique for Extraction of Hydrous Iron Oxides from Roots of Wetland Plants.
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Taylor, G.J., and Crowder, A.A. (1983). Use of the DCB Technique for Extraction of Hydrous Iron Oxides from Roots of Wetland Plants. American Journal of Botany, 70(8) 1254-1257
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Replicate samples of Phragmites communis roots coated with hydrous iron oxide were analyzed for internal and external Fe using the dithionite-citrate-bicarbonate technique (hot DCB), a modified DCB technique (cold DCB), Ethylenediaminete traacetic acid (EDTA), Diethylenetriamine pentaacetic acid (DTPA), and a total Fe analysis. EDTA and DTPA were found to be inefficient in extracting FeOOH while both DCB techniques were efficient. Concentrations of Fe extracted (8.93% and 9.37% by weight) and percent of total Fe extracted (98.3% and 98.6%) by the hot and cold DCB techniques (respectively) were not significantly different. Results of the total Fe analysis (8.58% Fe by weight) were the same as extracted Fe and total Fe determined by the DCB techniques. This may reflect the large external Fe: internal Fe ratio of the sample roots. Despite equivalent results the cold DCB technique is preferred over the hot DCB technique due to a reduced likelihood of structural damage to roots
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http://purl.org/coar/resource_type/c_6501 http://purl.org/coar/version/c_970fb48d4fbd8a85
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en