Roles of small RNAs in the effects of nutrition on apoptosis and spermatogenesis in the adult testis
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Guan, Yongjuan, Liang, Guanxiang, Hawken, Penelope A.R., Malecki, Irek A., Cozens, Greg, Vercoe, Philip E., Martin, Graeme B., & Guan, Le Luo. (2015). Roles of small RNAs in the effects of nutrition on apoptosis and spermatogenesis in the adult testis. Scientific Reports, 5, [14]. http://dx.doi.org/10.1038/srep10372
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http://dx.doi.org/10.1038/srep10372
Abstract
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We tested whether reductions in spermatozoal quality induced by under-nutrition are associated with increased germ cell apoptosis and disrupted spermatogenesis, and whether these effects are mediated by small RNAs. Groups of 8 male sheep were fed for a 10% increase or 10% decrease in body mass over 65 days. Underfeeding increased the number of apoptotic germ cells (P < 0.05) and increased the expression of apoptosis-related genes (P < 0.05) in testicular tissue. We identified 44 miRNAs and 35 putative piRNAs that were differentially expressed in well-fed and underfed males (FDR < 0.05). Some were related to reproductive system development, apoptosis (miRNAs), and sperm production and quality (piRNAs). Novel-miR-144 (miR-98), was found to target three apoptotic genes (TP53, CASP3, FASL). The proportion of miRNAs as a total of small RNAs was greater in well-fed males than in underfed males (P < 0.05) and was correlated (r = 0.8, P < 0.05) with the proportion of piRNAs in well-fed and underfed males. In conclusion, the reductions in spermatozoal quality induced by under-nutrition are caused, at least partly, by disruptions to Sertoli cell function and increased germ cell apoptosis, mediated by changes in the expression of miRNAs and piRNAs.
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http://purl.org/coar/resource_type/c_6501 http://purl.org/coar/version/c_970fb48d4fbd8a85
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Attribution 4.0 International
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en