Enzyme-assisted polymer film degradation-enabled biomolecule sensing with poly (N-isopropylacrylamide)-based optical devices

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dc.contributor.authorZhang, Wei
dc.contributor.authorWei, Menglian
dc.contributor.authorCarvalho, Wildemar S. P.
dc.contributor.authorSerpe, Michael J.
dc.date.accessioned2025-05-01T12:10:59Z
dc.date.available2025-05-01T12:10:59Z
dc.date.issued2018-01-01
dc.descriptionA biosensor for mouse Immunoglobulin G (IgG) was generated from responsive polymer-based interference filters (etalons). To accomplish this, an excess amount of alkaline phosphatase-modified goat anti-mouse IgG (AP-GAM, F(ab’)2 fragment specific to mouse IgG) was added to mouse IgG, and allowed to react for some time. After a given reaction time, the bound AP-GAM could be isolated from the unbound, excess AP-GAM by addition of goat anti-mouse IgG (Fc fragment specific)-modified magnetic microspheres (GAM-M) that bind the mouse IgG bound to AP-GAM. After application of a magnetic field, the free, unbound AP-GAM was isolated from the mixture and exposed to an etalon that has its upper Au surface modified with phosphate-containing polymer that can be degraded by AP-GAM. By the phosphate-containing polymer being degraded by the excess AP-GAM, the cleaved phosphate groups can diffuse into the interference filter's active polymer layer that yields a change in the optical properties that can be related to the amount of IgG in the sample. This concept is extremely straightforward to implement, and can be modified to detect a variety of other analytes of interest.
dc.identifier.doihttps://doi.org/10.7939/r3-pd9s-f424
dc.language.isoen
dc.rights© 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license. This is an open access article which is free to download and share.
dc.subjectSensing
dc.subjectPoly (N-Isopropylacrylamide)
dc.subjectBiosensing
dc.subjectEnzymatic Degradation
dc.subjectEtalons
dc.titleEnzyme-assisted polymer film degradation-enabled biomolecule sensing with poly (N-isopropylacrylamide)-based optical devices
dc.typehttp://purl.org/coar/resource_type/c_6501 http://purl.org/coar/version/c_b1a7d7d4d402bcce http://purl.org/coar/version/c_71e4c1898caa6e32
ual.jupiterAccesshttp://terms.library.ualberta.ca/public

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