Comparative proteomic and metabolomic analysis of a Staphylococcus variant (SG1) cultured in the presence and absence of butanol

Abstract

My research focuses on proteomic and metabolomic analysis of a solvent tolerant strain of Staphylococcus warneri called SG1 cultured in the presence and absence of 1-Butanol. On the proteomic analysis, the tryptic digests of SG1 were either directly analyzed by two-dimensional liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometry (2D-LC-ESI-QTOF MS) for protein identification or isotope labeled using dimethylation after guanidination (2-MEGA) followed by 2D-LC-ESI-QTOF MS for relative protein quantification. On the metabolomic analysis, the extracted metabolites were either labeled with 12C/13C dansyl chloride for relative quantitation of amine- and phenol-containing metabolites or labeled with 12C/13C p-dimethylaminophenacyl (DmPA) bromide for relative quantitation of carboxylic acid-containing metabolites. Finally, proteomic data and metabolomic data were combined and compared to help elucidate the solvent tolerant mechanism of SG1. The thesis work highlights the potential and significance of combining proteomic and metabolomic analyses for studying biological systems.