Fall 2025 theses and dissertations (non-restricted) will be available in ERA on November 17, 2025.

Identifying nonspecific ligand binding in electrospray ionization mass spectrometry using the reporter molecule method

Simple item page
dc.contributor.authorSun, Nian
dc.contributor.authorKlassen, John S.
dc.contributor.authorKitova, Elena N.
dc.contributor.authorSun, Jiangxiao
dc.date.accessioned2025-05-01T12:11:21Z
dc.date.available2025-05-01T12:11:21Z
dc.date.issued2009
dc.descriptionThe application of the reporter molecule (Mrep) method for identifying nonspecific complexes in the ES-MS analysis of protein-ligand and DNA-ligand interactions in vitro is described. To test the reliability of the method, it was applied to the ES-MS analysis of protein-carbohydrate complexes originating from specific interactions in solution and from nonspecific interactions in the ES process. These control experiments confirm the basic assumptions underlying the Mrep method, namely that nonspecific ligand binding is a random process, and that the ES droplet histories for specific and nonspecific complexes are distinct. The application of the Mrep method to the ES-MS analysis of the sequential binding of the ethidium cation, a DNA intercalator, to single and double strand oligodeoxynucleotides is also described, and highlights the general utility of the method.
dc.identifier.doihttps://doi.org/10.7939/R33B5WN5T
dc.language.isoen
dc.relationhttp://doi.org/10.1016/j.jasms.2009.02.024
dc.relation.isversionofSun, N., Sun, J., Kitova, E. N., & Klassen, J. S. (2009). Identifying nonspecific ligand binding in electrospray ionization mass spectrometry using the reporter molecule method. Journal of the American Society for Mass Spectrometry, 20(7), 1242-1250. http://doi.org/10.1016/j.jasms.2009.02.024
dc.rights© 2009 Sun, N., Sun, J., Kitova, E. N., & Klassen, J. S. This version of this article is open access and can be downloaded and shared. The original author(s) and source must be cited.
dc.subjectStability
dc.subjectNanoelectrospray ionization
dc.subjectAffinities
dc.subjectComplexes
dc.subjectEthidium-bromide
dc.subjectConstants
dc.subjectDNA
dc.subjectDissociation
dc.subjectNucleic acids
dc.subjectDuplex
dc.titleIdentifying nonspecific ligand binding in electrospray ionization mass spectrometry using the reporter molecule method
dc.typehttp://purl.org/coar/resource_type/c_6501 http://purl.org/coar/version/c_970fb48d4fbd8a85
ual.jupiterAccesshttp://terms.library.ualberta.ca/public

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
JASMS_20_7_1242.pdf
Size:
768.52 KB
Format:
Adobe Portable Document Format
Download

Collections

Journal Articles (Chemistry)