The Na+/H+ exchanger potentiates growth and retinoic acid induced differentiation of embryonal carcinoma cells
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Wang, Huayan, Singh, Dyal, & Fliegel, Larry. (1997). The Na+/H+ exchanger potentiates growth and retinoic acid induced differentiation of embryonal carcinoma cells. Journal of Biological Chemistry, 272(42), 26545-26549. https://doi.org/10.1074/jbc.272.42.26545
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https://doi.org/10.1074/jbc.272.42.26545
Abstract
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The Na+/H+exchanger is a ubiquitous plasma membrane protein that is responsible for pH regulation and is activated by growth factors. We examined the role of the Na+/H+ exchanger in cell growth and differentiation. Treatment of P19 cells with the Na+/H+ exchanger inhibitor Hoe 694 eliminated retinoic acid-induced differentiation in this cell line. We developed a P19 embryonal carcinoma cell line that was deficient in the Na+/H+ antiporter. Na+/H+ exchanger-deficient cells were reduced in the rate of cell growth and this effect was enhanced by the removal of added HCO3 − and by reducing extracellular pH. The antiporter-deficient cells were also markedly deficient in their ability to differentiate to neuronal-like cells and recovered this ability when the Na+/H+antiporter was reintroduced. The results show that the absence of Na+/H+ antiport as a pH regulatory mechanism can result in deficiencies in both cell growth and differentiation in embryonal carcinoma cells.
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http://purl.org/coar/resource_type/c_6501 http://purl.org/coar/version/c_970fb48d4fbd8a85
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en