Fall 2025 theses and dissertations (non-restricted) will be available in ERA on November 17, 2025.

Long-term catheterization of the intestinal lymph trunk and collection of lymph in neonatal pigs

Simple item page
dc.contributor.authorUwiera, Richard R.
dc.contributor.authorMangat, Rabban.
dc.contributor.authorKelly, Sandra
dc.contributor.authorUwiera, Trina C.
dc.contributor.authorProctor, Spencer D.
dc.date.accessioned2025-05-01T11:54:26Z
dc.date.available2025-05-01T11:54:26Z
dc.date.issued2016-01-01
dc.descriptionCatheterization of the intestinal lymph trunk in neonatal pigs is a technique allowing for the long-term collection of large quantities of intestinal (central) efferent lymph. Importantly, the collection of central lymph from the intestine enables researchers to study both the mechanisms and lipid constitutes associated with lipid metabolism, intestinal inflammation and cancer metastasis, as well as cells involved in immune function and immunosurveillance. A ventral mid-line surgical approach permits excellent surgical exposure to the cranial abdomen and relatively easy access to the intestinal lymph trunk vessel that lies near the pancreas and the right ventral segment of the portal vein underneath the visceral aspect of the right liver lobe. The vessel is meticulously dissected and released from the surrounding fascia and then dilated with sutures allowing for insertion and subsequent securing of the catheter into the vessel. The catheter is exteriorized and approximately 1 L/24 hr of lymph is collected over a 7 day period. While this technique enables the collection of large quantities of central lymph over an extended period of time, the success depends on careful surgical dissection, tissue handling and close attention to proper surgical technique. This is particularly important with surgeries in young animals as the lymph vessels can easily tear, potentially leading to surgical and experimental failure. The video demonstrates an excellent surgical technique for the collection of intestinal lymph.
dc.identifier.doihttps://doi.org/10.7939/r3-gsv4-8k20
dc.language.isoen
dc.relationhttp://dx.doi.org/10.3791/53457
dc.relation.isversionofUwiera, R. R. E., Mangat, R., Kelly, S., Uwiera, T. C., & Proctor, S. D. (2016). Long-term catheterization of the intestinal lymph trunk and collection of lymph in neonatal pigs. Journal of Visualized Experiments, 109, e53457. http://dx.doi.org/10.3791/53457
dc.rights© 2016 Uwiera, R. R. E., Mangat, R., Kelly, S., Uwiera, T. C., & Proctor, S. D. This version of this article is open access and can be downloaded and shared. The original author(s) and source must be cited.
dc.subjectIntestinal lymph trunk
dc.subjectLong-term
dc.subjectApoB lipoprotein
dc.subjectCatheterization
dc.subjectNeonatal pigs
dc.subjectEfferent lymph
dc.subjectLipoprotein metabolism
dc.subjectSurgery
dc.titleLong-term catheterization of the intestinal lymph trunk and collection of lymph in neonatal pigs
dc.typehttp://purl.org/coar/resource_type/c_6501 http://purl.org/coar/version/c_b1a7d7d4d402bcce http://purl.org/coar/version/c_71e4c1898caa6e32
ual.jupiterAccesshttp://terms.library.ualberta.ca/public

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
JVE_109_e53457.pdf
Size:
2.38 MB
Format:
Adobe Portable Document Format
Download

Collections

Journal Articles (Agricultural, Food and Nutritional Science)