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Chk1 and Wee1 kinases coordinate DNA replication, chromosome condensation and anaphase entry

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dc.contributor.authorCampbell, S.D.
dc.contributor.authorHomola, E.M.
dc.contributor.authorFasulo, B.
dc.contributor.authorYu, K.R.
dc.contributor.authorHsieh, T.S.
dc.contributor.authorSullivan, W.
dc.contributor.authorKoyama, C.
dc.date.accessioned2025-05-01T12:01:24Z
dc.date.available2025-05-01T12:01:24Z
dc.date.issued2012
dc.descriptionDefects in DNA replication and chromosome condensation are common phenotypes in cancer cells. A link between replication and condensation has been established, but little is known about the role of checkpoints in monitoring chromosome condensation. We investigate this function by live analysis, using the rapid division cycles in the early Drosophila embryo. We find that S-phase and topoisomerase inhibitors delay both the initiation and the rate of chromosome condensation. These cell cycle delays are mediated by the cell cycle kinases chk1 and wee1. Inhibitors that cause severe defects in chromosome condensation and congression on the metaphase plate result in delayed anaphase entry. These delays are mediated by wee1 and are not the result of spindle assembly checkpoint activation. In addition, we provide the first detailed live analysis of the direct effect of widely used anticancer agents (aclarubicin, ICRF-193, VM26, doxorubicin, camptothecin, aphidicolin, hydroxyurea, cisplatin, mechlorethamine and x-rays) on key nuclear and cytoplasmic cell cycle events.
dc.identifier.doihttps://doi.org/10.7939/R39P29
dc.language.isoen
dc.relation.isversionofB Fasulo, C Koyama, KR Yu, EM Homola, TS Hsieh, SD Campbell, W Sullivan. "Chk1 and Wee1 kinases coordinate DNA replication, chromosome condensation and anaphase entry." Mol Biol Cell. 2012 Jan 19.
dc.rights© 2012 Fasulo et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).
dc.subjectDrosophila-melanogaster
dc.subjectNuclear
dc.subjectPhosphorylation
dc.subjectTopoisomerase-II
dc.subjectPolo-like kinase-1
dc.subjectHuman-cells
dc.subjectDamage checkpoint
dc.subjectActivation
dc.subjectMitotic entry
dc.subjectEarly embryogenesis
dc.titleChk1 and Wee1 kinases coordinate DNA replication, chromosome condensation and anaphase entry
dc.typehttp://purl.org/coar/resource_type/c_6501 http://purl.org/coar/version/c_970fb48d4fbd8a85
ual.jupiterAccesshttp://terms.library.ualberta.ca/public

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