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Contribution of glutaminases to glutamine metabolism and acid resistance in Lactobacillus reuteri and other vertebrate host adapted lactobacilli

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dc.contributor.authorLi, Qing
dc.contributor.authorTao, QianYing
dc.contributor.authorTeixeira, Juanana S.
dc.contributor.authorShu-Wei Su, Marcia
dc.contributor.authorGänzle, Michael G.
dc.date.accessioned2025-05-01T11:54:00Z
dc.date.available2025-05-01T11:54:00Z
dc.date.issued2020-04-01
dc.descriptionThe bacterial conversion of glutamine to glutamate is catalyzed by glutamine-amidotransferases or glutaminases. Glutamine deamination contributes to the formation of the bioactive metabolites glutamate, γ-aminobutyrate (GABA) and γ-glutamyl peptides, and to acid resistance. This study aimed to investigate the distribution of glutaminase(s) in lactobacilli, and to evaluate their contribution in L. reuteri to amino acid metabolism and acid resistance. Phylogenetic analysis of the glutaminases gls1, gls2 and gls3 in the genus Lactobacillus demonstrated that glutaminase is exclusively present in host-adapted species of lactobacilli. The disruption gls1, gls2 and gls3 in L. reuteri 100-23 had only a limited effect on the conversion of glutamine to glutamate, GABA, or γ-glutamyl peptides in sourdough. The disruption of all glutaminases in L. reuteri 100-23Δgls1Δgls2Δgls3 but not disruption of gls2 and gls3 eliminated the protective effect of glutamine on the survival of the strain at pH 2.5. Glutamine also enhanced acid resistance of L. reuteri 100-23ΔgadB and L. taiwanensis 107q, strains without glutamate decarboxylase activity. Taken together, the study demonstrates that glutaminases of lactobacilli do not contribute substantially to glutamine metabolism but enhance acid resistance. Their exclusive presence in host-adapted lactobacilli provides an additional link between the adaptation of lactobacilli to specific habitats and their functionality when used as probiotics and starter cultures.
dc.identifier.doihttps://doi.org/10.7939/r3-r3ct-8j70
dc.language.isoen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectLactobacillus reuteri
dc.subjectGlutaminase
dc.subjectIntestinal lactobacilli
dc.subjectAcid resistance
dc.subjectSourdough
dc.titleContribution of glutaminases to glutamine metabolism and acid resistance in Lactobacillus reuteri and other vertebrate host adapted lactobacilli
dc.typehttp://purl.org/coar/resource_type/c_6501 http://purl.org/coar/version/c_b1a7d7d4d402bcce http://purl.org/coar/version/c_71e4c1898caa6e32
dcterms.sourceLi, Q., Tao, Q., Teixeira, J. S., Shu-Wei Su, M., & Gänzle, M. G. (2020). Contribution of glutaminases to glutamine metabolism and acid resistance in Lactobacillus reuteri and other vertebrate host adapted lactobacilli. Food Microbiology, 86. https://doi-org.login.ezproxy.library.ualberta.ca/10.1016/j.fm.2019.103343
ual.jupiterAccesshttp://terms.library.ualberta.ca/public

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