The effect of vinorelbine on MCF-7 and MDA-MB-231 human breast cancer cell lines
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Vinorelbine, otherwise known as Navelbine or KW-2307, is a semi-synthesized chemotherapeutic drug part of the class of compounds known as Vinca alkaloids. Like other Vinca alkaloids, vinorelbine interacts with microtubules, specifically non-polymerized monomeric tubulin proteins. This interaction prevents the polymerization and subsequent assembly of the microtubules, thereby arresting cells in the G2/metaphase stage. Vinorelbine is commonly used in treating breast cancers and non-small cell lung cancers (NSCLC). The aim of this research is to investigate the mode of cell death caused by vinorelbine in MDA-MB-231 versus MCF-7 human breast cancer cell lines, which are metastatic and non-metastatic respectively, and to evaluate whether metastasis plays a significant role in the drug interaction. Three XTT growth inhibition assays were performed on each cell line in the presence and absence of vinorelbine after 24 or 48 hours, cell death was visualized by DAPI staining each cell line in the presence and absence of vinorelbine after 24 or 48 hours, and Western blotting was used to analyze the mode of cell death in the presence and absence of vinorelbine after 24 or 48 hours by determining the presence of Beclin, LC3, and Caspase-3 proteins.
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http://purl.org/coar/resource_type/c_1843
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en