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Characterization of α-Galactooligosaccharides formed via Heterologous Expression of α- 2 Galactosidases from Lactobacillus reuteri in Lactococcus lactis

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dc.contributor.authorWang, Yvonne
dc.contributor.authorBlack, Brenna A.
dc.contributor.authorCurtis, Jonathan M.
dc.contributor.authorGänzle, Michael G.
dc.date.accessioned2025-05-01T11:54:27Z
dc.date.available2025-05-01T11:54:27Z
dc.date.issued2014-01-01
dc.descriptionα-Galacto-oligosaccharides (α-GOS) are produced by transgalactosylation reactions of α-galactosidase (α-Gal) or by conversion of raffinose family oligosaccharides by levansucrase. Similarly to β-GOS, α-GOS have the potential to mimic glycan receptors on eukaryotic cells and act as molecular decoys to prevent bacterial infection; however, data on transgalactosylation reactions of α-Gal remain scarce. The α-Gal gene sequence from Lactobacillus reuteri was cloned into an α-Gal negative strain of Lactococcus lactis. Transgalactosylation reactions were achieved using crude cell extracts with melibiose or raffinose as galactosyl donor and fucose, N-acetylglucosamine or lactose as galactosyl acceptor. The composition, sequence and most linkage types of α-GOS formed with acceptors saccharides were determined by liquid chromatography-tandem mass spectrometry. α-Gal of Lactobacillus reuteri formed (1 → 3)-, (1 → 4)- or (1 → 6)-linked α-GOS but exhibited a preference for formation of (1 → 6)-linkages. Fucose, N-acetylglucosamine and lactose were suitable galactosyl acceptors for α-Gal of L. reuteri, resulting in formation of (1 → 3)-, (1 → 4)- or (1 → 6)-linked hetero-oligosaccharides. By determining the structural specificity of α-Gal and increasing the variation of oligosaccharides produced by introducing alternative acceptor sugars, this work supports further studies to assess α-GOS pathogen adhesion prevention in mammalian hosts.
dc.identifier.doihttps://doi.org/10.7939/r3-xr8c-yx17
dc.language.isoen
dc.relationhttp://dx.doi.org/10.1007/s00253-013-5145-x
dc.relation.isversionofWang, Y., Black, B., Curtis, J.M., & Gänzle, M.G. (2014). Characterization of α-galactooligosaccharides formed via heterologous expression of α-Galactosidases from Lactobacillus reuteri in Lactococcus lactis. Applied Microbiology and Biotechnology, 98(6), 2507-2517. http://dx.doi.org/10.1007/s00253-013-5145-x
dc.rights© Springer-Verlag Berlin Heidelberg 2013
dc.subjectLactobacillus reuteri
dc.subjectgalacto-oligosaccharides
dc.subjecthetero-oligosaccharides
dc.subjectα-galactosidase
dc.subjectLC/MS
dc.titleCharacterization of α-Galactooligosaccharides formed via Heterologous Expression of α- 2 Galactosidases from Lactobacillus reuteri in Lactococcus lactis
dc.typehttp://purl.org/coar/resource_type/c_6501 http://purl.org/coar/version/c_b1a7d7d4d402bcce http://purl.org/coar/version/c_71e4c1898caa6e32
ual.jupiterAccesshttp://terms.library.ualberta.ca/public

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