Metabolism of isomalto‐oligosaccharides by Lactobacillus reuteri and bifidobacteria

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dc.contributor.authorHu, Y.
dc.contributor.authorKetabi, A.
dc.contributor.authorBuchko, A.
dc.contributor.authorGänzle, Michael G.
dc.date.accessioned2025-05-01T11:54:11Z
dc.date.available2025-05-01T11:54:11Z
dc.date.issued2013-08-01
dc.descriptionCommercial isomalto‐oligosaccharides (IMO) are functional food ingredients. They are composed of α(1→6)‐ and α(1→4)‐linked oligosaccharides. IMO are partially indigestible, and dietary IMO stimulate beneficial members of intestinal microbiota, including lactobacilli and bifidobacteria. However, data on IMO metabolism by lactobacilli are not available. It was the aim of this study to identify metabolic pathways of IMO metabolism in lactobacilli. This study focused on the host‐adapted species Lactobacillus reuteri. Metabolism of bifidobacteria was analysed for comparison. Commercial IMO contained IMO with a degree of polymerization (DP) of up to four and panose‐series oligosaccharides (POS) with a DP of up to 5. Lactobacilli metabolized isomaltose preferentially over oligosaccharides with higher DP. Bifidobacteria preferentially metabolized oligosaccharides with higher DP and accumulated glucose. Metabolism of IMO and POS by L. reuteri was attributed to α(1→6)‐specific glucanase DexB and maltose phosphorylase. Contribution of maltose phosphorylase was verified by quantification of IMO and POS phosphorolysis in crude cellular extracts of L. reuteri 100‐23. In conclusion, metabolism of IMO by lactobacilli is limited to short‐chain oligosaccharides, while bifidobacteria preferentially metabolize oligosaccharides with higher DP. The functionality of commercial IMO can thus be modified by degree of polymerization.Isomalto‐oligosaccharides (IMO) are applied as functional food ingredients, but the composition and biological functionality of current commercial products are poorly documented. This study is the first to analyse IMO metabolism by Lactobacillus reuteri. Bifidobacteria were used for comparison. Commercial IMO contained IMO with degree of polymerization (DP) of up to four and panose‐series oligosaccharides with DP of up to 5. L. reuteri preferentially metabolized short‐chain oligosaccharides, whereas bifidobacteria preferentially metabolized higher oligosaccharides. Results of this study allow the modification of the biological and technological functionality of commercial IMO by adjustment of the degree of polymerization and will thus facilitate the application development for IMO.
dc.identifier.doihttps://doi.org/10.7939/r3-3v0p-yr98
dc.language.isoen
dc.relationhttps://doi.org/10.1111/lam.12076
dc.relation.isversionofHu, Y., Ketabi, A., Buchko, A., & Gänzle, M. G. (2013). Metabolism of isomalto‐oligosaccharides by Lactobacillus reuteri and bifidobacteria. Letters in Applied Microbiology, 57(2), 108–114. https://doi.org/10.1111/lam.12076
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectLactobacillus
dc.subjectBifidobacterium
dc.subjectIsomalto-oligosaccharides (IMO)
dc.subjectMaltose phosphorylase
dc.titleMetabolism of isomalto‐oligosaccharides by Lactobacillus reuteri and bifidobacteria
dc.typehttp://purl.org/coar/resource_type/c_6501 http://purl.org/coar/version/c_b1a7d7d4d402bcce http://purl.org/coar/version/c_71e4c1898caa6e32
dcterms.sourceThis article has been accepted for publication in Letters in Applied Microbiology published by Oxford University Press.
ual.jupiterAccesshttp://terms.library.ualberta.ca/public

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