A Cells Capture, Separation and Detection Modelling Method in the Microfluidic and IDEs Based Biosensor

Abstract

Lab-on-a-Chip technologies have been developed over the past few decades and it has a wide range of applications. For example, in the field of medicine and agriculture, portable devices with the biosensoring function have a great potential in disease detection for animals and crop disease prevention for plants. To capture the targeted cells from real and complicated biological samples, dielectrophoresis (DEP) was proposed. A DEP force could be employed as a label-free technology for achieving target cell capture and separation. Target cells can then be detected and counted using impedance spectroscopy. To prevent the targets from being drafted away during the measurements, the frequency range of DEP should overlap the impedance measurement frequency. In this study, we use a sample interdigitated electrode configuration to explore the impacts of DEP force, and also to figure out the feasible frequency range during the capture, separation, and detection of cells in the microfluidic system. Simulations provide a modelling method and guidance of a kinetics system in the microfluidic channel.