Microbial community development and function at a newly reclaimed oil sands site
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Abstract
Assessment of microbial community development is required to determine the success of reclamation process on disturbed land after mining. Peat (PMM) or LFH mineral soil mix (LFH) is used as capping material in reclamation. Application of coarse woody debris (CWD) also facilitates reclamation by developing microsites for biogeochemical processes. To investigate the effect of reclamation material on microbial activities and functions, a study was conducted over a 3-year period at a reclaimed site in Fort MacMurray, Canada. Soil samples were collected bi-annually from LFH and PMM plots amended with or without CWD. Results revealed an increasing trend over time in microbial biomass carbon and nitrogen, respiration rate, β-glucosidase and N-acetylglucosaminidase enzyme activities, and abundances of functional genes (amoA, nirS and nifH) involved in nitrogen cycle. LFH showed more pronounced effect than PMM due to its higher decomposed organic matter content. Application of CWD and addition of fresh labile carbon inputs in fall enhanced microbial growth and function.